Science
Microbiology
Department Profile
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Teaching Staff
Dr.Sharada Beknal |
Dr.Sumangala Deshmukh |
Courses & Syllabus
Microbiology is a fascinating branch of biology with applications in several fields such as biotechnology, molecular biology, medicine, nanotechnology, bioinformatics, agriculture and industry. With the advent of recombinant DNA technology, researches on microbiology have enabled us to produce transgenic plants, animals, fungi and bacteria with new genetic traits. Conceptually, the way one studies living organisms has changed in fundamental ways as there seems to be very little difference between microbes and higher organisms at the functional level. The behavior of basic molecules of life such as nucleic acids, proteins, carbohydrates, and lipids seem to be the same and these molecules are interchangeable in different organisms.
As a result of the development of molecular biology and biotechnology, new fields of specialization known as bioethics, biosafety, environmental impacts, biological patents, and intellectual property rights have evolved. Public perceptions and acceptance of the applications of the knowledge of molecular biology, will be to a large extent influenced by ethics, cultural moores, and safety of the products of biotechnology. There is now a hot debate underway to restrict research funding in some areas that are fundamentally changing the methods of reproduction through nuclear transformation and cloning in animal systems with potential application in human cloning. The students of biology are well advised to be alert to these developments in order to shape their professional careers. Since understanding of microorganisms has advanced considerably during the last 15-20 years there have been many changes in higher education with microbiology being and important component of college and university curriculum in applied biology.
Programme specific outcomes for B.Sc Microbiology
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Sl. no |
Program |
Program Objectives |
Program specific Objectives |
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1 |
B.Sc Microbiology |
Since Microbiology is one the applied subjects that impart knowledge in different aspects of microbiology for individual and society also. The vast research over world wide for the battle of various diseases caused by microbes. The microbes were exploited for various applications ex. Production of food and its safety measures, as biocontrol agents, medicines, agriculture, environmental fields. Microbiological tools have been extensively used to study different life process with various technologies like r-DNA technologies, DNA finger printing technologies, bioinformatics, fermentation technology, nanotechnology and industry with career opportunities at undergraduate level. |
First year B.Sc: students will study basic concepts of microbiology with introduction to microbiological world. Exposure and study of different microscopes, instruments and isolation and observation of microorganisms with their morphological, staining, cultivation, and classification of microorganisms. Second year B.Sc: students will be given necessary information on the microbes involved in different metabolism and microbial genetics. Third year B.Sc: students are exposed to broad applied areas of microbiology like food microbiology, soil microbiology, medical microbiology, industrial microbiology. Overall objectives: to enhance and train the students to different microbial techniques and exposure to handle different types of microscopes. To encourage more students to opt P.G courses and research activities. Career opportunities for students to built up in various fields of microbiology ex. Fermentation technology, cultivation of mushrooms etc. |
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Sl.no |
Course |
Couse Outcomes |
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First year B.Sc |
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1 |
First year B.Sc paper 1.1 General Microbiology |
1. Students will understand the basic concepts of microbiology and different branches of microbiology. 2. Exposure to simple, compound and different types of microscopes. 3. Study and differentiate between different types of microorganisms i.e, prokaryotic and eukaryotic microorganisms and their classifications. |
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2 |
2.1 Microbial Techniques |
1. Sterilization, instrumentation and their working principles. 2. Stains and staining techniques, different types of media. 3. Isolation and enumeration of pure cultures |
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First year B.Sc practicals 1.1 and 2.1
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Able to understand the principal and handling of microscopes. Study of different microorganisms with permanent slides. Studying the motility of organisms.
Preparation of different types of media, cultivation and staining of microorganisms and identifying the morphological features of microorganisms with the help of microscopes. Able to understand the working principles of different instruments. Cultivation of microorganisms as pure cultures. |
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Second year B.Sc
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4. |
3.1 Microbial metabolism
4.1 Microbial Genetics |
1.Students are able to understand the concept of enzyme activities calssification and factor influencing enzyme activity. 2.They will be able to study the importance of nutritrional requirements and types of bacteria,microbial growth and factors influencing microbial growth and growth curve. 3.Students will be able to illustrate about various metabolic pathways like EMP cycle,TCA,ED pathway, Glyoxylate cycle and Beta oxidation cycle. 4.They will be able to understand the concept of formation and respiration. 5. They will be able to describe the importance of photosynthesis in microorganisms with special reference to purple and green bacteria. 1. They will be able to summarize the basic concept of genetics i.e, DNA and RNA structure, gene, central dogma of gene structure and protein synthesis. 2. They will be able to explain about mutation and different types of mutational changes and their causes. 3. Transfer of genetic material through process of recombination, transformation, transduction and conjugation. 4. Study on tools used in genetic engineering and applications of genetic engineering with suitable examples, clones and cloning techniques. |
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Second year B.Sc practicals 3.1
4.1 |
Measurement of growth of microorganisms using hemocytometer. Study of IMVIC tests, Biochemical tests, growth curve, qualitative analysis of microorganisms by serial dilution followed by plate count method. Cultivation of anaerobic microorganisms. Estimation of sugars, proteins by biochemical methods. Extraction of DNA Using house hold articles. Demonstration of recombination, transformation, transduction and conjugation and protoplast fusion Tchniques. Isolation of streptomycin resistant microorganisms. Exposure of microorganisms to UV light and study its mutational characters.
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Third year B.Sc |
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6. |
Third year B.Sc paper 5.1 Food Microbiology
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1.Students will be able to understand the role of microorganisms in food and dairy products. 2.They will be able to describe the microorganisms involved in food contamination, spoilage of food and preservation. 3. Role of microorganisms in fermented foods ex. Idli, yoghurt, cheese etc, single cell protein and single cell oil. 4. Food sanitation. |
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5.2 Soil Microbiology |
1. Students will be able to understand the different types of microorganisms present in soil, soil profile and soil fertility. 2. Role of microorganisms in Rhizosphere, Phyllosphere and Mycorhizae and their interactions with plants, microorganisms as plant pathogens. 3. Relation of microorganisms in transformation of Carbon, Nitrogen, Sulphur and Phosphorous in soil. 4. They will be able to understand the application of biofertilizers, biopesticides in agriculture. 5. microorganisms involved in relation to biodegradation in soil, Nitrogen fixation by microorganisms. |
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6.1 Medical Microbiology |
1. Students will be able to understand the term Immunology, types of immunity, components of immune systems, Immunoglobulins, Antigen-Antibody interactions. 2. They will be able to classify and characterize disease causing organisms like bacterial, viral and fungal. 3. Characterization of Vaccines(Bacterial, Fungal). |
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6.2 Industrial Microbiology |
1. Microorganisms used in industries, nature of raw materials, source and its importance in production. 2. They will be able to understand the different types of fermentation process. 3. Industrial production of Alcohol, Organic acids, Solvents and Antibiotics. 4. Waste water treatment. |
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Third year B.Sc practicals 5.1
5.2
6.1
6.2 |
Iolation of microorganisms from curds, rotten fruits and vegetables. Counting of microorganisms in milk samples. Assessment of milk to test the quality by methylene blue reduction test. Estimation of lactic acid from curd sample. Isolation, observation and cultivation of microorganisms from soil samples. Examination of bacteroids from root nodules. Observation of plant pathogens. Estimation of soil alkanity. Students will be trained with various techniques in clinical microbiology- blood sample, urine and stool examination, sputum sample for AFB, immumohematology, ELISA test, VDRL test, WIDAL test, Antibiotic sensitivity test etc. Microscopic observation of human pathogens and isolation using swab culture. Observation of industrially important microorganisms. Estimation of Alcohol percentage. Report of various microorganisms in sewage water sample, Coliform test for drinking water. Immobilization techniques. |
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Research
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Activities
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Program & Course Outcome
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Alumni List
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Future Plans
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